Journal: Journal of Translational Medicine
Article Title: Intra-tumoural vessel area estimated by expression of epidermal growth factor-like domain 7 and microRNA-126 in primary tumours and metastases of patients with colorectal cancer: a descriptive study
doi: 10.1186/s12967-014-0359-y
Figure Lengend Snippet: Visualisation of EGFL7 and miRNA-126. Representative images of (A) : the intra-tumoural expression of Epidermal Growth Factor-like Domain 7 (EGFL7), by immunohistochemical analysis, showing staining of endothelial cells (ECs, brown) and the corresponding classified image (Ac) demonstrating intense staining of ECs (green) and faint staining (dark green) of myofibroblasts. Only the intense signal was included in the quantitative estimate. (B) The microRNA-126 (miRNA-126) in situ hybridization (ISH) analysis, showing merely exclusive intra-tumoural expression of ECs (blue) and the corresponding classified image (Bc) demonstrating the ECs included in the quantitative estimate (green).
Article Snippet: 4 μl recombinant EGFL7 (Novus Biologicals H00051162-P01) and 1 μl of the anti-EGFL7 antibody were mixed in 200 μl dilution buffer (Dako EnVision FLEX WASH BUFFER K8007) to give approximately 2.4 μM antigen versus 0.6 μM antibody.
Techniques: Expressing, Immunohistochemical staining, Staining, In Situ Hybridization